Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1944327..1944940 Replicon chromosome
Accession NC_021740
Organism Mycobacterium tuberculosis EAI5

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag M943_RS09050 Protein ID WP_003408465.1
Coordinates 1944327..1944716 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag M943_RS09055 Protein ID WP_003408469.1
Coordinates 1944713..1944940 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M943_RS09020 1939956..1940870 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
M943_RS09025 1940873..1941703 + 831 WP_003916848.1 cyclase family protein -
M943_RS09030 1941703..1942053 + 351 WP_003898986.1 cupin domain-containing protein -
M943_RS09035 1942106..1942924 + 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
M943_RS09040 1942938..1943717 + 780 WP_003408460.1 IclR family transcriptional regulator -
M943_RS09050 1944327..1944716 - 390 WP_003408465.1 PIN domain-containing protein Toxin
M943_RS09055 1944713..1944940 - 228 WP_003408469.1 antitoxin Antitoxin
M943_RS09060 1945158..1946642 + 1485 WP_003904684.1 biotin carboxylase -
M943_RS09065 1946639..1947886 + 1248 WP_003408476.1 serine hydrolase -
M943_RS09070 1947929..1948348 - 420 WP_003904685.1 hypothetical protein -
M943_RS09075 1948338..1949048 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T29728 WP_003408465.1 NC_021740:c1944716-1944327 [Mycobacterium tuberculosis EAI5]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T29728 NC_021740:c1944716-1944327 [Mycobacterium tuberculosis EAI5]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT29728 WP_003408469.1 NC_021740:c1944940-1944713 [Mycobacterium tuberculosis EAI5]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT29728 NC_021740:c1944940-1944713 [Mycobacterium tuberculosis EAI5]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References