Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1683570..1684186 Replicon chromosome
Accession NC_021740
Organism Mycobacterium tuberculosis EAI5

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag M943_RS07900 Protein ID WP_003407593.1
Coordinates 1683869..1684186 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag M943_RS07895 Protein ID WP_003900349.1
Coordinates 1683570..1683872 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M943_RS07885 1679456..1681303 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
M943_RS07890 1681304..1683556 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
M943_RS07895 1683570..1683872 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
M943_RS07900 1683869..1684186 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
M943_RS07905 1684183..1685187 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
M943_RS07910 1685240..1686529 + 1290 WP_003407599.1 serine hydrolase -
M943_RS07915 1686602..1687375 - 774 WP_003912632.1 class I SAM-dependent methyltransferase -
M943_RS07920 1687433..1687645 - 213 WP_003898900.1 dodecin family protein -
M943_RS22265 1687655..1687879 - 225 WP_160522526.1 NUDIX domain-containing protein -
M943_RS07930 1688149..1689177 + 1029 WP_003903478.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T29726 WP_003407593.1 NC_021740:1683869-1684186 [Mycobacterium tuberculosis EAI5]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T29726 NC_021740:1683869-1684186 [Mycobacterium tuberculosis EAI5]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT29726 WP_003900349.1 NC_021740:1683570-1683872 [Mycobacterium tuberculosis EAI5]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT29726 NC_021740:1683570-1683872 [Mycobacterium tuberculosis EAI5]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References