Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 157134..157777 Replicon plasmid P1
Accession NZ_OY019089
Organism Escherichia coli O25b:H4-ST131 isolate 42

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag QU406_RS26310 Protein ID WP_001034044.1
Coordinates 157361..157777 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag QU406_RS26305 Protein ID WP_001261286.1
Coordinates 157134..157364 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QU406_RS26285 (153765) 153765..154520 - 756 WP_000852146.1 replication initiation protein RepE -
QU406_RS26290 (155242) 155242..156048 - 807 WP_000016970.1 site-specific integrase -
QU406_RS26295 (156049) 156049..156354 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
QU406_RS26300 (156356) 156356..156574 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
QU406_RS26305 (157134) 157134..157364 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
QU406_RS26310 (157361) 157361..157777 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
QU406_RS26315 (157852) 157852..159411 + 1560 Protein_192 AAA family ATPase -
QU406_RS26325 (160181) 160181..160821 + 641 Protein_194 NADPH-dependent L-lysine N(6)-monooxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / qacE / sul1 / mph(A) / tet(A) senB / iucD / iutA 1..168227 168227


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T297226 WP_001034044.1 NZ_OY019089:157361-157777 [Escherichia coli O25b:H4-ST131]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT297226 WP_001261286.1 NZ_OY019089:157134-157364 [Escherichia coli O25b:H4-ST131]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References