Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3656704..3657280 Replicon chromosome
Accession NZ_OY019088
Organism Escherichia coli O25b:H4-ST131 isolate 42

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag QU406_RS17770 Protein ID WP_001295743.1
Coordinates 3656704..3656991 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag QU406_RS17775 Protein ID WP_000063148.1
Coordinates 3656978..3657280 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QU406_RS17755 (3652364) 3652364..3653668 - 1305 WP_000535012.1 restriction endonuclease subunit S -
QU406_RS17760 (3653658) 3653658..3655277 - 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
QU406_RS17765 (3655341) 3655341..3656507 - 1167 WP_000800831.1 restriction endonuclease -
QU406_RS17770 (3656704) 3656704..3656991 + 288 WP_001295743.1 BrnT family toxin Toxin
QU406_RS17775 (3656978) 3656978..3657280 + 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
QU406_RS17780 (3657314) 3657314..3658270 - 957 WP_001295745.1 GTPase -
QU406_RS17785 (3658281) 3658281..3658484 - 204 WP_000467859.1 YbdD/YjiX family protein -
QU406_RS17790 (3658534) 3658534..3660684 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T297210 WP_001295743.1 NZ_OY019088:3656704-3656991 [Escherichia coli O25b:H4-ST131]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT297210 WP_000063148.1 NZ_OY019088:3656978-3657280 [Escherichia coli O25b:H4-ST131]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References