Detailed information of TA system TA029721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1238065..1238633
• Replicon: chromosome
• Accession: NC_021740
• Organism: Mycobacterium tuberculosis EAI5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: M943_RS05900
• Protein ID: WP_003405865.1
• Coordinates: 1238259..1238633 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: M943_RS05895
• Protein ID: WP_003405863.1
• Coordinates: 1238065..1238262 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M943_RS05875	1234106..1234744	-	639	WP_003898730.1	lipid droplet-associated protein	-
M943_RS05880	1234834..1235841	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
M943_RS05885	1235858..1236841	-	984	WP_020824483.1	hypothetical protein	-
M943_RS05890	1236904..1237977	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
M943_RS05895	1238065..1238262	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
M943_RS05900	1238259..1238633	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
M943_RS05905	1238836..1239534	+	699	WP_003898733.1	hypothetical protein	-
M943_RS05910	1239652..1239837	+	186	WP_003901093.1	hypothetical protein	-
M943_RS05915	1239764..1240039	-	276	WP_003405867.1	hypothetical protein	-
M943_RS05920	1240282..1240605	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
M943_RS05925	1240620..1241330	-	711	WP_003911399.1	hypothetical protein	-
M943_RS22415	1241352..1241561	+	210	WP_003911400.1	hypothetical protein	-
M943_RS05935	1241513..1242220	-	708	Protein_1158	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T29721 WP_003405865.1 NC_021740:1238259-1238633 [Mycobacterium tuberculosis EAI5]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T29721 NC_021740:1238259-1238633 [Mycobacterium tuberculosis EAI5]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT29721 WP_003405863.1 NC_021740:1238065-1238262 [Mycobacterium tuberculosis EAI5]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT29721 NC_021740:1238065-1238262 [Mycobacterium tuberculosis EAI5]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07