Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1229309..1229940 Replicon chromosome
Accession NC_021740
Organism Mycobacterium tuberculosis EAI5

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag M943_RS05840 Protein ID WP_003405820.1
Coordinates 1229309..1229620 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag M943_RS05845 Protein ID WP_003405836.1
Coordinates 1229620..1229940 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M943_RS05820 1224790..1226214 - 1425 WP_003405805.1 class II fumarate hydratase -
M943_RS05825 1226245..1227333 - 1089 WP_017351234.1 class II fructose-bisphosphatase -
M943_RS05830 1227389..1228033 + 645 WP_003915494.1 DUF4245 domain-containing protein -
M943_RS05835 1228040..1229197 - 1158 WP_015631268.1 AI-2E family transporter -
M943_RS05840 1229309..1229620 - 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
M943_RS05845 1229620..1229940 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
M943_RS20895 1229950..1231475 + 1526 Protein_1142 carboxylesterase/lipase family protein -
M943_RS05860 1231493..1232605 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
M943_RS05865 1232615..1232872 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
M943_RS05870 1232862..1234109 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
M943_RS05875 1234106..1234744 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T29720 WP_003405820.1 NC_021740:c1229620-1229309 [Mycobacterium tuberculosis EAI5]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T29720 NC_021740:c1229620-1229309 [Mycobacterium tuberculosis EAI5]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT29720 WP_003405836.1 NC_021740:c1229940-1229620 [Mycobacterium tuberculosis EAI5]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT29720 NC_021740:c1229940-1229620 [Mycobacterium tuberculosis EAI5]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References