Detailed information of TA system TA029717

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1013332..1013951
• Replicon: chromosome
• Accession: NC_021740
• Organism: Mycobacterium tuberculosis EAI5

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: M943_RS04785
• Protein ID: WP_003404726.1
• Coordinates: 1013517..1013951 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: G0TFQ5
• Locus tag: M943_RS04780
• Protein ID: WP_003404724.1
• Coordinates: 1013332..1013511 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M943_RS04770	1008805..1010385	+	1581	WP_015629748.1	serine hydrolase	-
M943_RS04775	1010382..1012775	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
M943_RS22370	1013048..1013206	+	159	WP_003404720.1	hypothetical protein	-
M943_RS04780	1013332..1013511	+	180	WP_003404724.1	antitoxin	Antitoxin
M943_RS04785	1013517..1013951	+	435	WP_003404726.1	SRPBCC family protein	Toxin
M943_RS04790	1014049..1014822	+	774	WP_003404735.1	VOC family protein	-
M943_RS04795	1014887..1015336	+	450	WP_003404738.1	hypothetical protein	-
M943_RS04800	1015471..1015701	-	231	WP_003898642.1	hypothetical protein	-
M943_RS04805	1015868..1017376	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
M943_RS04810	1017378..1018616	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T29717 WP_003404726.1 NC_021740:1013517-1013951 [Mycobacterium tuberculosis EAI5]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T29717 NC_021740:1013517-1013951 [Mycobacterium tuberculosis EAI5]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6394.06 Da
• Isoelectric Point: 4.3059

>AT29717 WP_003404724.1 NC_021740:1013332-1013511 [Mycobacterium tuberculosis EAI5]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLQDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT29717 NC_021740:1013332-1013511 [Mycobacterium tuberculosis EAI5]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCAGGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: G0TFQ5