Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 114693..115218 Replicon plasmid P1
Accession NZ_OX637965
Organism Escherichia coli O25b:H4-ST131 isolate 46

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag QQG90_RS26360 Protein ID WP_001159868.1
Coordinates 114693..114998 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag QQG90_RS26365 Protein ID WP_000813634.1
Coordinates 115000..115218 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQG90_RS26345 (110603) 110603..111769 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
QQG90_RS26350 (112357) 112357..113112 - 756 WP_000852146.1 replication initiation protein RepE -
QQG90_RS26355 (113886) 113886..114692 - 807 WP_000016982.1 site-specific integrase -
QQG90_RS26360 (114693) 114693..114998 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
QQG90_RS26365 (115000) 115000..115218 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
QQG90_RS26370 (115798) 115798..116886 + 1089 WP_000952217.1 transcriptional repressor PifC -
QQG90_RS26375 (116888) 116888..119113 + 2226 WP_000698737.1 P-loop NTPase fold protein -
QQG90_RS26380 (119163) 119163..120062 - 900 WP_000963206.1 nucleotide-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA17 / aadA5 / qacE / sul1 senB 1..123938 123938


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T297165 WP_001159868.1 NZ_OX637965:c114998-114693 [Escherichia coli O25b:H4-ST131]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT297165 WP_000813634.1 NZ_OX637965:c115218-115000 [Escherichia coli O25b:H4-ST131]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References