Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 127558..128083 Replicon plasmid P1
Accession NZ_OX637962
Organism Escherichia coli O25b:H4-ST131 isolate 86

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag QQG89_RS24620 Protein ID WP_001159868.1
Coordinates 127558..127863 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag QQG89_RS24625 Protein ID WP_000813634.1
Coordinates 127865..128083 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQG89_RS24605 (123468) 123468..124634 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
QQG89_RS24610 (125222) 125222..125977 - 756 WP_000852146.1 replication initiation protein RepE -
QQG89_RS24615 (126751) 126751..127557 - 807 WP_000016982.1 site-specific integrase -
QQG89_RS24620 (127558) 127558..127863 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
QQG89_RS24625 (127865) 127865..128083 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
QQG89_RS24630 (128791) 128791..129786 + 996 WP_000246636.1 hypothetical protein -
QQG89_RS24635 (129790) 129790..130722 + 933 WP_000991832.1 S-4TM family putative pore-forming effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-27 senB 1..146919 146919
- flank IS/Tn - - 130859..131362 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T297137 WP_001159868.1 NZ_OX637962:c127863-127558 [Escherichia coli O25b:H4-ST131]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT297137 WP_000813634.1 NZ_OX637962:c128083-127865 [Escherichia coli O25b:H4-ST131]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References