Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4098949..4099525 Replicon chromosome
Accession NZ_OX637961
Organism Escherichia coli O25b:H4-ST131 isolate 86

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QQG89_RS20000 Protein ID WP_001297643.1
Coordinates 4099238..4099525 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QQG89_RS19995 Protein ID WP_000063156.1
Coordinates 4098949..4099251 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQG89_RS19980 (4095545) 4095545..4097695 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QQG89_RS19985 (4097745) 4097745..4097948 + 204 WP_000467859.1 YbdD/YjiX family protein -
QQG89_RS19990 (4097959) 4097959..4098915 + 957 WP_001297640.1 GTPase -
QQG89_RS19995 (4098949) 4098949..4099251 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QQG89_RS20000 (4099238) 4099238..4099525 - 288 WP_001297643.1 BrnT family toxin Toxin
QQG89_RS20005 (4099724) 4099724..4100421 - 698 Protein_3920 IS1 family transposase -
QQG89_RS20010 (4100478) 4100478..4100573 + 96 Protein_3921 dienelactone hydrolase family protein -
QQG89_RS20015 (4100719) 4100719..4101951 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
QQG89_RS20020 (4101992) 4101992..4103272 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T297127 WP_001297643.1 NZ_OX637961:c4099525-4099238 [Escherichia coli O25b:H4-ST131]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT297127 WP_000063156.1 NZ_OX637961:c4099251-4098949 [Escherichia coli O25b:H4-ST131]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References