Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4102828..4103404 Replicon chromosome
Accession NZ_OX637959
Organism Escherichia coli O25b:H4-ST131 isolate 32

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QQG88_RS20025 Protein ID WP_001297643.1
Coordinates 4103117..4103404 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QQG88_RS20020 Protein ID WP_000063156.1
Coordinates 4102828..4103130 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQG88_RS20005 (4099424) 4099424..4101574 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QQG88_RS20010 (4101624) 4101624..4101827 + 204 WP_000467859.1 YbdD/YjiX family protein -
QQG88_RS20015 (4101838) 4101838..4102794 + 957 WP_001297640.1 GTPase -
QQG88_RS20020 (4102828) 4102828..4103130 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QQG88_RS20025 (4103117) 4103117..4103404 - 288 WP_001297643.1 BrnT family toxin Toxin
QQG88_RS20030 (4103603) 4103603..4104300 - 698 Protein_3925 IS1 family transposase -
QQG88_RS20035 (4104357) 4104357..4104452 + 96 Protein_3926 dienelactone hydrolase family protein -
QQG88_RS20040 (4104598) 4104598..4105830 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
QQG88_RS20045 (4105871) 4105871..4107151 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T297100 WP_001297643.1 NZ_OX637959:c4103404-4103117 [Escherichia coli O25b:H4-ST131]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT297100 WP_000063156.1 NZ_OX637959:c4103130-4102828 [Escherichia coli O25b:H4-ST131]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References