Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 716421..717070 Replicon chromosome
Accession NC_021740
Organism Mycobacterium tuberculosis EAI5

Toxin (Protein)


Gene name vapC Uniprot ID P67241
Locus tag M943_RS03265 Protein ID WP_003403236.1
Coordinates 716675..717070 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ34
Locus tag M943_RS03260 Protein ID WP_003403235.1
Coordinates 716421..716675 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M943_RS22245 711546..711629 + 84 Protein_639 galactose-1-phosphate uridylyltransferase -
M943_RS03235 711648..712730 + 1083 WP_031652152.1 galactose-1-phosphate uridylyltransferase -
M943_RS03240 712727..713818 + 1092 WP_003900977.1 galactokinase -
M943_RS03250 714213..715370 + 1158 WP_003903091.1 hypothetical protein -
M943_RS03255 715381..716328 + 948 WP_003403232.1 DUF732 domain-containing protein -
M943_RS03260 716421..716675 + 255 WP_003403235.1 type II toxin-antitoxin system antitoxin VapB30 Antitoxin
M943_RS03265 716675..717070 + 396 WP_003403236.1 type II toxin-antitoxin system toxin ribonuclease C30 Toxin
M943_RS03270 717164..717904 - 741 WP_003403239.1 TVP38/TMEM64 family protein -
M943_RS03275 718036..718296 + 261 WP_003403244.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
M943_RS03280 718293..718700 + 408 WP_003403246.1 type II toxin-antitoxin system VapC family toxin -
M943_RS03285 718772..719923 - 1152 WP_003403248.1 FIST C-terminal domain-containing protein -
M943_RS03290 720016..721743 - 1728 WP_003900979.1 exodeoxyribonuclease V subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

Antitoxin VapB-like

(1-83)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14250.04 Da        Isoelectric Point: 4.7475

>T29710 WP_003403236.1 NC_021740:716675-717070 [Mycobacterium tuberculosis EAI5]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Download         Length: 396 bp

>T29710 NC_021740:716675-717070 [Mycobacterium tuberculosis EAI5]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9139.52 Da        Isoelectric Point: 9.2702

>AT29710 WP_003403235.1 NC_021740:716421-716675 [Mycobacterium tuberculosis EAI5]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Download         Length: 255 bp

>AT29710 NC_021740:716421-716675 [Mycobacterium tuberculosis EAI5]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4XGR
PDB 4XGQ

Antitoxin

Source ID Structure
PDB 4XGR
PDB 4XGQ
AlphaFold DB A0A7U4BSC2

References