Detailed information of TA system TA029707

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 697164..697810
• Replicon: chromosome
• Accession: NC_021740
• Organism: Mycobacterium tuberculosis EAI5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: M943_RS03120
• Protein ID: WP_003403137.1
• Coordinates: 697164..697577 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: M943_RS03125
• Protein ID: WP_003403139.1
• Coordinates: 697574..697810 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M943_RS03100	693247..694797	+	1551	WP_003403119.1	MCE family protein	-
M943_RS03105	694849..695241	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
M943_RS03110	695238..695495	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
M943_RS03115	695678..696913	-	1236	WP_003403128.1	ATP-binding protein	-
M943_RS03120	697164..697577	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
M943_RS03125	697574..697810	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
M943_RS03130	697914..698420	-	507	WP_003900973.1	two component system sensor kinase HK1	-
M943_RS03135	698534..699064	-	531	WP_003917320.1	two component system sensor kinase HK2	-
M943_RS03140	699048..699743	-	696	WP_200861092.1	response regulator transcription factor	-
M943_RS03145	699866..700177	+	312	WP_003403164.1	hypothetical protein	-
M943_RS03150	700249..701199	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
M943_RS03155	701440..702024	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
M943_RS03160	702026..702736	+	711	Protein_625	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T29707 WP_003403137.1 NC_021740:c697577-697164 [Mycobacterium tuberculosis EAI5]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T29707 NC_021740:c697577-697164 [Mycobacterium tuberculosis EAI5]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT29707 WP_003403139.1 NC_021740:c697810-697574 [Mycobacterium tuberculosis EAI5]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT29707 NC_021740:c697810-697574 [Mycobacterium tuberculosis EAI5]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89