Detailed information of TA system TA297053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 68006..69735
• Replicon: chromosome
• Accession: NZ_OX461105
• Organism: Serratia proteamaculans strain EBP3064 isolate EBP3064

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: QOT07_RS00300
• Protein ID: WP_261417782.1
• Coordinates: 68006..68662 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: QOT07_RS00305
• Protein ID: WP_261417783.1
• Coordinates: 68659..69735 (+)
• Length: 359 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QOT07_RS00280	63302..63907	+	606	WP_085115218.1	glutathione S-transferase	-
QOT07_RS00285	64122..65513	+	1392	WP_283602389.1	L-seryl-tRNA(Sec) selenium transferase	-
QOT07_RS00290	65510..67351	+	1842	WP_283602390.1	selenocysteine-specific translation elongation factor	-
QOT07_RS00295	67426..68019	+	594	WP_261417780.1	DUF4433 domain-containing protein	-
QOT07_RS00300	68006..68662	+	657	WP_261417782.1	DUF4433 domain-containing protein	Toxin
QOT07_RS00305	68659..69735	+	1077	WP_261417783.1	macro domain-containing protein	Antitoxin
QOT07_RS00310	69775..71022	-	1248	WP_262261822.1	MFS transporter	-
QOT07_RS00315	71242..72234	+	993	WP_283602391.1	acyltransferase	-
QOT07_RS00320	72229..73152	-	924	WP_283602392.1	DJ-1/PfpI family protein	-
QOT07_RS00325	73155..73706	-	552	WP_085115225.1	isochorismatase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (15-212)

Antitoxin

• Macro domain (18-131)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25056.59 Da
• Isoelectric Point: 8.4554

>T297053 WP_261417782.1 NZ_OX461105:68006-68662 [Serratia proteamaculans]
MANYKSLNPQKALIWRIVHKDNLPWILANGLHAGSSEKKASDWVSIGNTELIERRKHRKVPVAPGGTLDDYIPFYFTPFS
PMLYNIHTGRGGVARQANEDIVILVSSLHRLQQQERVFVFTDRHAYTPMAGYYSHLAQLDKIDWELLQQRNFNRVPDKPE
QIERYQAEALVHHHLPVTALLGIVCYSSALQEKLQKEFGHLLNIQCLPDWYFNGGGNQ

Nucleotide

• Length: 657 bp

Antitoxin

Protein

• Length: 359 a.a.
• Molecular weight: 40601.05 Da
• Isoelectric Point: 7.0442

>AT297053 WP_261417783.1 NZ_OX461105:68659-69735 [Serratia proteamaculans]
MIRFTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPANMKAYSAACKAETVQTGKMFVTDTGELMGPRWIINFPTK
QHWRSPSKLEWIVEGLRDLRRFIMQNNIASIAIPPLGAGHGGLDWVAVRDVIIQELSDLPQVDIIIYEPIEKYQNVAKSV
GVEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERAIIAQQVENPLNLQFKAQNYGPYADRLRHLLNGLDGSYLR
CEKRLGDAKPLDIIWFNDAKRDVVLAYLKSEAKTWLPALQKTTELIDGFESPYGLELLSTIDWLVYQEHISADEESLAEG
IRHWPAGERWAQRKSGLFDKTAINMALTRLQTLYPPHA

Nucleotide

• Length: 1077 bp