297041

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1293048..1293965 Replicon chromosome
Accession NZ_OX460973
Organism Bacillus velezensis strain SAF3325 substr. 0 isolate SAF3325

Toxin (Protein)


Gene name spoIISA Uniprot ID I2HQ15
Locus tag QOS64_RS06715 Protein ID WP_007407256.1
Coordinates 1293219..1293965 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag QOS64_RS06710 Protein ID WP_003154807.1
Coordinates 1293048..1293218 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QOS64_RS06675 (1288281) 1288281..1289903 + 1623 WP_076423972.1 pyocin knob domain-containing protein -
QOS64_RS06680 (1289916) 1289916..1290287 + 372 WP_136396299.1 XkdW family protein -
QOS64_RS06685 (1290292) 1290292..1290489 + 198 WP_007610833.1 XkdX family protein -
QOS64_RS06690 (1290546) 1290546..1291307 + 762 WP_015239693.1 hypothetical protein -
QOS64_RS06695 (1291359) 1291359..1291622 + 264 WP_003154815.1 hemolysin XhlA family protein -
QOS64_RS06700 (1291636) 1291636..1291899 + 264 WP_003154813.1 phage holin -
QOS64_RS06705 (1291913) 1291913..1292791 + 879 WP_043021264.1 N-acetylmuramoyl-L-alanine amidase -
QOS64_RS06710 (1293048) 1293048..1293218 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
QOS64_RS06715 (1293219) 1293219..1293965 - 747 WP_007407256.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
QOS64_RS06720 (1294070) 1294070..1295068 - 999 WP_007407255.1 inorganic phosphate transporter -
QOS64_RS06725 (1295081) 1295081..1295698 - 618 WP_003154804.1 DUF47 domain-containing protein -
QOS64_RS06730 (1295984) 1295984..1297300 - 1317 WP_007610842.1 amino acid permease -
QOS64_RS06735 (1297625) 1297625..1298575 + 951 WP_012117369.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29077.57 Da        Isoelectric Point: 4.7755

>T297041 WP_007407256.1 NZ_OX460973:c1293965-1293219 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT297041 WP_003154807.1 NZ_OX460973:c1293218-1293048 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB I2HQ15

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References