Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 499824..500461 Replicon chromosome
Accession NZ_OX460973
Organism Bacillus velezensis strain SAF3325 substr. 0 isolate SAF3325

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag QOS64_RS02520 Protein ID WP_003156187.1
Coordinates 500111..500461 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag QOS64_RS02515 Protein ID WP_003156188.1
Coordinates 499824..500105 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QOS64_RS02495 (496189) 496189..496788 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
QOS64_RS02500 (496881) 496881..497246 + 366 WP_094031521.1 holo-ACP synthase -
QOS64_RS02505 (497411) 497411..498418 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
QOS64_RS02510 (498535) 498535..499704 + 1170 WP_098081026.1 alanine racemase -
QOS64_RS02515 (499824) 499824..500105 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
QOS64_RS02520 (500111) 500111..500461 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
QOS64_RS02525 (500579) 500579..501400 + 822 WP_003156182.1 STAS domain-containing protein -
QOS64_RS02530 (501405) 501405..501770 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
QOS64_RS02535 (501773) 501773..502174 + 402 WP_003156178.1 anti-sigma regulatory factor -
QOS64_RS02540 (502186) 502186..503193 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
QOS64_RS02545 (503257) 503257..503586 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
QOS64_RS02550 (503583) 503583..504065 + 483 WP_003156173.1 anti-sigma B factor RsbW -
QOS64_RS02555 (504031) 504031..504819 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
QOS64_RS02560 (504819) 504819..505421 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T297040 WP_003156187.1 NZ_OX460973:500111-500461 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT297040 WP_003156188.1 NZ_OX460973:499824-500105 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References