Detailed information of TA system TA029697

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 2108101..2108751
• Replicon: chromosome
• Accession: NC_021739
• Organism: Mannheimia haemolytica D174

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J451_RS10745
• Protein ID: WP_020824375.1
• Coordinates: 2108362..2108751 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A547EDM1
• Locus tag: J451_RS10740
• Protein ID: WP_006249412.1
• Coordinates: 2108101..2108361 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J451_RS10725	2103789..2107322	+	3534	WP_006253629.1	transcription-repair coupling factor	-
J451_RS10730	2107485..2107772	+	288	WP_006251764.1	BrnT family toxin	-
J451_RS10735	2107773..2107985	+	213	WP_006251765.1	BrnA antitoxin family protein	-
J451_RS10740	2108101..2108361	+	261	WP_006249412.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
J451_RS10745	2108362..2108751	+	390	WP_020824375.1	type II toxin-antitoxin system VapC family toxin	Toxin
J451_RS10750	2108771..2110009	+	1239	WP_006253630.1	peptidase T	-
J451_RS10780	2110752..2111537	+	786	WP_006251768.1	hypothetical protein	-
J451_RS10785	2111611..2112471	+	861	WP_006250124.1	metal ABC transporter permease	-
J451_RS10790	2112464..2113273	+	810	WP_006250123.1	metal ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-119)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14614.86 Da
• Isoelectric Point: 6.7020

>T29697 WP_020824375.1 NC_021739:2108362-2108751 [Mannheimia haemolytica D174]
MYMLDTNTVSYFFRGNANVVAKLKSINPERLCISSVTAAELVYGVAKRNNGQLSQFLDYFLSSVKVLDWNYHCAELYGKL
RAEMEKNGKVMGVQDQMIASHALAEECILVSSDQAFKMVPDLKLENWLH

Nucleotide

• Length: 390 bp

>T29697 NC_021739:2108362-2108751 [Mannheimia haemolytica D174]
ATGTATATGTTAGATACGAATACTGTAAGCTATTTTTTTCGTGGAAATGCAAATGTGGTGGCGAAATTGAAGTCCATTAA
TCCTGAGAGGCTTTGTATTTCATCTGTAACTGCTGCTGAATTGGTTTATGGTGTGGCGAAAAGAAATAATGGGCAGCTTT
CTCAATTTTTAGATTATTTTTTATCGAGTGTAAAGGTTTTAGATTGGAATTATCATTGTGCTGAACTTTATGGAAAGTTA
CGGGCAGAGATGGAGAAAAATGGCAAAGTAATGGGCGTGCAGGATCAAATGATTGCTAGTCACGCTCTTGCTGAAGAGTG
TATTTTAGTTTCTAGTGATCAGGCATTTAAAATGGTACCTGATCTGAAATTGGAGAATTGGTTACACTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10245.54 Da
• Isoelectric Point: 4.8951

>AT29697 WP_006249412.1 NC_021739:2108101-2108361 [Mannheimia haemolytica D174]
MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKKENGDILLSLRSRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQR
DPFEVA

Nucleotide

• Length: 261 bp

>AT29697 NC_021739:2108101-2108361 [Mannheimia haemolytica D174]
ATGGAAAAAATTGCGAGATTATTCCGTAACGGGCGGAACCAAGCAGTGCGTTTACCGGTTGAATTTGAGTTTGATGCAGA
GCAAGTCTATGTTTCTAAAAAAGAAAATGGCGATATTTTATTATCATTGAGATCGAGAAATACCGAGCATTGGCAACGAT
TGTTTGAGCTATTGCCTGCTGTTGTATGTGATGAAACATTTTTAGATACAAAAGAGAGAAACCAATCCTTTGAGCAACGG
GATCCTTTTGAGGTAGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A547EDM1