Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 564578..565214 Replicon chromosome
Accession NZ_OX419652
Organism Bacillus subtilis isolate NRS6131

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag KJP56_RS02980 Protein ID WP_003156187.1
Coordinates 564864..565214 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag KJP56_RS02975 Protein ID WP_003225183.1
Coordinates 564578..564859 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJP56_RS02955 (560937) 560937..561536 - 600 WP_041850724.1 rhomboid family intramembrane serine protease -
KJP56_RS02960 (561631) 561631..561996 + 366 WP_015252768.1 holo-ACP synthase -
KJP56_RS02965 (562162) 562162..563178 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
KJP56_RS02970 (563293) 563293..564462 + 1170 WP_015252766.1 alanine racemase -
KJP56_RS02975 (564578) 564578..564859 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
KJP56_RS02980 (564864) 564864..565214 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KJP56_RS02985 (565330) 565330..566154 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
KJP56_RS02990 (566159) 566159..566524 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
KJP56_RS02995 (566528) 566528..566929 + 402 WP_003225192.1 serine/threonine-protein kinase RsbT -
KJP56_RS03000 (566941) 566941..567948 + 1008 WP_213419561.1 PP2C family protein-serine/threonine phosphatase -
KJP56_RS03005 (568017) 568017..568346 + 330 WP_014475877.1 anti-sigma factor antagonist RsbV -
KJP56_RS03010 (568343) 568343..568825 + 483 WP_021481702.1 anti-sigma B factor RsbW -
KJP56_RS03015 (568791) 568791..569579 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
KJP56_RS03020 (569579) 569579..570178 + 600 WP_095252677.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T296949 WP_003156187.1 NZ_OX419652:564864-565214 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT296949 WP_003225183.1 NZ_OX419652:564578-564859 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References