Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1396973..1397889 Replicon chromosome
Accession NZ_OX419580
Organism Bacillus subtilis isolate NRS6116

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag KJP60_RS07465 Protein ID WP_003244695.1
Coordinates 1397143..1397889 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag KJP60_RS07460 Protein ID WP_003232646.1
Coordinates 1396973..1397143 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJP60_RS07425 (1393836) 1393836..1394165 + 330 WP_003232660.1 XkdW family protein -
KJP60_RS07430 (1394162) 1394162..1394326 + 165 WP_003232658.1 XkdX family protein -
KJP60_RS07435 (1394370) 1394370..1395209 + 840 WP_003245597.1 phage-like element PBSX protein XepA -
KJP60_RS07440 (1395262) 1395262..1395531 + 270 WP_003232655.1 hemolysin XhlA family protein -
KJP60_RS07445 (1395544) 1395544..1395807 + 264 WP_003232653.1 phage holin -
KJP60_RS07450 (1395820) 1395820..1396713 + 894 WP_003245230.1 N-acetylmuramoyl-L-alanine amidase -
KJP60_RS07455 (1396750) 1396750..1396887 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
KJP60_RS07460 (1396973) 1396973..1397143 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
KJP60_RS07465 (1397143) 1397143..1397889 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
KJP60_RS07470 (1397999) 1397999..1399000 - 1002 WP_003232642.1 inorganic phosphate transporter -
KJP60_RS07475 (1399013) 1399013..1399630 - 618 WP_003218470.1 DUF47 domain-containing protein -
KJP60_RS07480 (1399906) 1399906..1401222 - 1317 WP_003244819.1 serine/threonine exchanger -
KJP60_RS07485 (1401611) 1401611..1402561 + 951 WP_003245086.1 ring-cleaving dioxygenase -
KJP60_RS07490 (1402662) 1402662..1402808 + 147 WP_003244977.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1362981..1406309 43328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T296929 WP_003244695.1 NZ_OX419580:c1397889-1397143 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT296929 WP_003232646.1 NZ_OX419580:c1397143-1396973 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References