Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 567185..567821 Replicon chromosome
Accession NZ_OX419580
Organism Bacillus subtilis isolate NRS6116

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag KJP60_RS03015 Protein ID WP_003156187.1
Coordinates 567471..567821 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag KJP60_RS03010 Protein ID WP_003225183.1
Coordinates 567185..567466 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJP60_RS02990 (563544) 563544..564143 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
KJP60_RS02995 (564238) 564238..564603 + 366 WP_003234281.1 holo-ACP synthase -
KJP60_RS03000 (564769) 564769..565785 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
KJP60_RS03005 (565900) 565900..567069 + 1170 WP_003234284.1 alanine racemase -
KJP60_RS03010 (567185) 567185..567466 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
KJP60_RS03015 (567471) 567471..567821 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KJP60_RS03020 (567936) 567936..568760 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
KJP60_RS03025 (568765) 568765..569130 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
KJP60_RS03030 (569134) 569134..569535 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
KJP60_RS03035 (569547) 569547..570554 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
KJP60_RS03040 (570616) 570616..570945 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
KJP60_RS03045 (570942) 570942..571424 + 483 WP_003234299.1 anti-sigma B factor RsbW -
KJP60_RS03050 (571390) 571390..572178 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
KJP60_RS03055 (572178) 572178..572777 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T296928 WP_003156187.1 NZ_OX419580:567471-567821 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT296928 WP_003225183.1 NZ_OX419580:567185-567466 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References