Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 467838..468486 Replicon chromosome
Accession NC_021739
Organism Mannheimia haemolytica D174

Toxin (Protein)


Gene name hicA Uniprot ID A0A249A099
Locus tag J451_RS02335 Protein ID WP_006251221.1
Coordinates 467838..468014 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A248ZZL6
Locus tag J451_RS02340 Protein ID WP_006251222.1
Coordinates 468070..468486 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J451_RS02310 463156..465651 - 2496 WP_006251216.1 phage tail length tape measure family protein -
J451_RS02320 466196..466654 - 459 WP_006251218.1 hypothetical protein -
J451_RS02325 466823..467056 + 234 WP_020824316.1 hypothetical protein -
J451_RS02330 467071..467742 - 672 WP_006251220.1 hypothetical protein -
J451_RS02335 467838..468014 + 177 WP_006251221.1 type II toxin-antitoxin system HicA family toxin Toxin
J451_RS02340 468070..468486 + 417 WP_006251222.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
J451_RS02345 468526..469008 - 483 WP_006251223.1 phage tail protein -
J451_RS02350 469012..469392 - 381 WP_006251224.1 hypothetical protein -
J451_RS02355 469389..469757 - 369 WP_006251225.1 hypothetical protein -
J451_RS02360 469759..470103 - 345 WP_006251226.1 hypothetical protein -
J451_RS02365 470103..470480 - 378 WP_006251227.1 hypothetical protein -
J451_RS02370 470483..470743 - 261 WP_020824317.1 hypothetical protein -
J451_RS02375 470755..471744 - 990 WP_006251229.1 encapsulin -
J451_RS02380 471759..472193 - 435 WP_006251230.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 451001..517432 66431


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-64)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6640.79 Da        Isoelectric Point: 10.7708

>T29692 WP_006251221.1 NC_021739:467838-468014 [Mannheimia haemolytica D174]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Download         Length: 177 bp

>T29692 NC_021739:467838-468014 [Mannheimia haemolytica D174]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15519.91 Da        Isoelectric Point: 4.3508

>AT29692 WP_006251222.1 NC_021739:468070-468486 [Mannheimia haemolytica D174]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Download         Length: 417 bp

>AT29692 NC_021739:468070-468486 [Mannheimia haemolytica D174]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A099

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZZL6

References