Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 516722..517358 Replicon chromosome
Accession NZ_OX419579
Organism Bacillus subtilis isolate NRS6103

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag KJP43_RS02640 Protein ID WP_003156187.1
Coordinates 517008..517358 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag KJP43_RS02635 Protein ID WP_003225183.1
Coordinates 516722..517003 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJP43_RS02615 (513082) 513082..513681 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
KJP43_RS02620 (513776) 513776..514141 + 366 WP_015252768.1 holo-ACP synthase -
KJP43_RS02625 (514307) 514307..515323 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
KJP43_RS02630 (515437) 515437..516606 + 1170 WP_029727188.1 alanine racemase -
KJP43_RS02635 (516722) 516722..517003 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
KJP43_RS02640 (517008) 517008..517358 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KJP43_RS02645 (517473) 517473..518297 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
KJP43_RS02650 (518302) 518302..518667 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
KJP43_RS02655 (518671) 518671..519072 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
KJP43_RS02660 (519084) 519084..520091 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
KJP43_RS02665 (520153) 520153..520482 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
KJP43_RS02670 (520479) 520479..520961 + 483 WP_003234299.1 anti-sigma B factor RsbW -
KJP43_RS02675 (520927) 520927..521715 + 789 WP_029727187.1 RNA polymerase sigma factor SigB -
KJP43_RS02680 (521715) 521715..522314 + 600 WP_003234303.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T296913 WP_003156187.1 NZ_OX419579:517008-517358 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT296913 WP_003225183.1 NZ_OX419579:516722-517003 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References