Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 513320..513956 Replicon chromosome
Accession NZ_OX419578
Organism Bacillus subtilis isolate NRS6128

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag KJP45_RS02630 Protein ID WP_003156187.1
Coordinates 513606..513956 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag KJP45_RS02625 Protein ID WP_003225183.1
Coordinates 513320..513601 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJP45_RS02605 (NRS6128_02580) 509679..510278 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
KJP45_RS02610 (NRS6128_02585) 510373..510738 + 366 WP_014475874.1 holo-ACP synthase -
KJP45_RS02615 (NRS6128_02590) 510904..511920 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
KJP45_RS02620 (NRS6128_02595) 512035..513204 + 1170 WP_213414867.1 alanine racemase -
KJP45_RS02625 (NRS6128_02600) 513320..513601 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
KJP45_RS02630 (NRS6128_02605) 513606..513956 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KJP45_RS02635 (NRS6128_02610) 514071..514895 + 825 WP_015252765.1 RsbT co-antagonist protein RsbRA -
KJP45_RS02640 (NRS6128_02615) 514900..515265 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
KJP45_RS02645 (NRS6128_02620) 515269..515670 + 402 WP_017697001.1 serine/threonine-protein kinase RsbT -
KJP45_RS02650 (NRS6128_02625) 515682..516689 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
KJP45_RS02655 (NRS6128_02630) 516751..517080 + 330 WP_213414866.1 anti-sigma factor antagonist -
KJP45_RS02660 (NRS6128_02635) 517077..517559 + 483 WP_003234299.1 anti-sigma B factor RsbW -
KJP45_RS02665 (NRS6128_02640) 517525..518313 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
KJP45_RS02670 (NRS6128_02645) 518313..518912 + 600 WP_003234303.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T296911 WP_003156187.1 NZ_OX419578:513606-513956 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT296911 WP_003225183.1 NZ_OX419578:513320-513601 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References