Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 519968..520604 Replicon chromosome
Accession NZ_OX419577
Organism Bacillus subtilis isolate NRS6120

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag KJP47_RS02665 Protein ID WP_003156187.1
Coordinates 520254..520604 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag KJP47_RS02660 Protein ID WP_003225183.1
Coordinates 519968..520249 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJP47_RS02640 (516328) 516328..516927 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
KJP47_RS02645 (517022) 517022..517387 + 366 WP_014478897.1 holo-ACP synthase -
KJP47_RS02650 (517553) 517553..518569 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
KJP47_RS02655 (518683) 518683..519852 + 1170 WP_014478898.1 alanine racemase -
KJP47_RS02660 (519968) 519968..520249 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
KJP47_RS02665 (520254) 520254..520604 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KJP47_RS02670 (520709) 520709..521533 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
KJP47_RS02675 (521538) 521538..521903 + 366 WP_043858089.1 RsbT antagonist protein RsbS -
KJP47_RS02680 (521907) 521907..522308 + 402 WP_017697001.1 serine/threonine-protein kinase RsbT -
KJP47_RS02685 (522320) 522320..523327 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
KJP47_RS02690 (523389) 523389..523718 + 330 WP_014662855.1 anti-sigma factor antagonist RsbV -
KJP47_RS02695 (523715) 523715..524197 + 483 WP_003234299.1 anti-sigma B factor RsbW -
KJP47_RS02700 (524163) 524163..524951 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
KJP47_RS02705 (524951) 524951..525550 + 600 WP_003234303.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T296900 WP_003156187.1 NZ_OX419577:520254-520604 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT296900 WP_003225183.1 NZ_OX419577:519968-520249 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References