Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 518253..518889 Replicon chromosome
Accession NZ_OX419575
Organism Bacillus subtilis isolate NRS6121

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag KJP52_RS02650 Protein ID WP_003156187.1
Coordinates 518539..518889 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag KJP52_RS02645 Protein ID WP_003225183.1
Coordinates 518253..518534 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJP52_RS02625 (514612) 514612..515211 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
KJP52_RS02630 (515306) 515306..515671 + 366 WP_003234281.1 holo-ACP synthase -
KJP52_RS02635 (515837) 515837..516853 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
KJP52_RS02640 (516968) 516968..518137 + 1170 WP_003234284.1 alanine racemase -
KJP52_RS02645 (518253) 518253..518534 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
KJP52_RS02650 (518539) 518539..518889 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KJP52_RS02655 (519004) 519004..519828 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
KJP52_RS02660 (519833) 519833..520198 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
KJP52_RS02665 (520202) 520202..520603 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
KJP52_RS02670 (520615) 520615..521622 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
KJP52_RS02675 (521684) 521684..522013 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
KJP52_RS02680 (522010) 522010..522492 + 483 WP_003234299.1 anti-sigma B factor RsbW -
KJP52_RS02685 (522458) 522458..523246 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
KJP52_RS02690 (523246) 523246..523845 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T296888 WP_003156187.1 NZ_OX419575:518539-518889 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT296888 WP_003225183.1 NZ_OX419575:518253-518534 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References