Detailed information of TA system TA029688

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 1450520..1451235
• Replicon: chromosome
• Accession: NC_021738
• Organism: Mannheimia haemolytica D171

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J450_RS07110
• Protein ID: WP_006250491.1
• Coordinates: 1450807..1451235 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A248ZW87
• Locus tag: J450_RS07105
• Protein ID: WP_006248272.1
• Coordinates: 1450520..1450807 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J450_RS07090	1446359..1448074	-	1716	WP_006248268.1	proline--tRNA ligase	-
J450_RS07095	1448238..1448420	-	183	WP_006250493.1	hypothetical protein	-
J450_RS07100	1448473..1450356	-	1884	WP_006250492.1	molecular chaperone HtpG	-
J450_RS07105	1450520..1450807	+	288	WP_006248272.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
J450_RS07110	1450807..1451235	+	429	WP_006250491.1	type II toxin-antitoxin system VapC family toxin	Toxin
J450_RS07115	1451239..1453320	+	2082	WP_006250490.1	ATP-dependent DNA helicase RecG	-
J450_RS07120	1453342..1454274	-	933	WP_006250489.1	nucleoside hydrolase	-
J450_RS07125	1454327..1455979	-	1653	WP_006250488.1	phospho-sugar mutase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-129)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 16640.06 Da
• Isoelectric Point: 8.9269

>T29688 WP_006250491.1 NC_021738:1450807-1451235 [Mannheimia haemolytica D171]
MYRYLFDTNIVSELYKLRNDKMDVNVRQWLRGVNPSQTNISCITLSEIKTGILLKARKDKEQSDRLNDWFENNVLKAYQT
KAFVVNNDIALLASEYHIPNKMDLNDAYIAATAKYHNLILVTRNTKDFIRCDVRLFNPFETA

Nucleotide

• Length: 429 bp

>T29688 NC_021738:1450807-1451235 [Mannheimia haemolytica D171]
ATGTATCGCTACCTTTTTGATACCAATATAGTTTCCGAGCTTTATAAGTTAAGAAATGACAAGATGGATGTCAATGTTCG
CCAGTGGCTGAGAGGGGTTAATCCTAGCCAGACCAATATCAGCTGTATTACGTTATCTGAAATCAAAACAGGTATTTTGC
TGAAAGCCCGCAAAGACAAAGAACAATCAGACCGATTAAATGATTGGTTTGAAAATAATGTATTAAAGGCTTATCAAACA
AAAGCGTTTGTGGTGAATAACGACATTGCTCTATTGGCATCAGAGTACCATATTCCCAATAAAATGGATTTAAATGATGC
TTATATTGCGGCAACCGCCAAATACCACAATCTGATTTTAGTCACCCGCAACACCAAAGATTTTATCCGTTGTGATGTGC
GTTTATTTAACCCATTTGAAACCGCATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11085.39 Da
• Isoelectric Point: 4.3648

>AT29688 WP_006248272.1 NC_021738:1450520-1450807 [Mannheimia haemolytica D171]
MAITITSREFNQNASKVLKMAEKEPVFITKWGKIVNVVSSYMDYQQKKPSEPSFEELFASSENEDVSDEFWQEFEQTLVE
IRKSNKPRPVDFGDE

Nucleotide

• Length: 288 bp

>AT29688 NC_021738:1450520-1450807 [Mannheimia haemolytica D171]
ATGGCAATAACCATTACTTCCAGAGAATTTAATCAAAATGCCAGCAAAGTGTTAAAAATGGCAGAAAAAGAGCCGGTTTT
TATTACCAAATGGGGCAAAATCGTCAATGTGGTGTCTAGTTATATGGATTATCAGCAGAAAAAGCCTAGCGAGCCAAGCT
TTGAAGAGCTATTCGCCTCTTCCGAAAATGAGGATGTGAGCGATGAATTTTGGCAAGAGTTTGAACAAACATTGGTTGAA
ATCCGCAAAAGCAATAAACCAAGACCTGTTGATTTTGGAGATGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A248ZW87