Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1226144..1226733 Replicon chromosome
Accession NC_021738
Organism Mannheimia haemolytica D171

Toxin (Protein)


Gene name pemK Uniprot ID A0A248ZY19
Locus tag J450_RS05940 Protein ID WP_006248516.1
Coordinates 1226401..1226733 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A248ZX26
Locus tag J450_RS05935 Protein ID WP_006248517.1
Coordinates 1226144..1226404 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J450_RS05910 1221323..1222468 - 1146 WP_006251102.1 FMN-dependent L-lactate dehydrogenase LldD -
J450_RS05915 1223080..1223724 + 645 WP_006248521.1 RNA pyrophosphohydrolase -
J450_RS05920 1223726..1224514 + 789 WP_006248520.1 sulfite exporter TauE/SafE family protein -
J450_RS05925 1224522..1225319 + 798 WP_006251103.1 prolipoprotein diacylglyceryl transferase -
J450_RS05930 1225406..1226074 + 669 WP_006248518.1 phosphoglycolate phosphatase -
J450_RS05935 1226144..1226404 + 261 WP_006248517.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
J450_RS05940 1226401..1226733 + 333 WP_006248516.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
J450_RS05945 1226860..1227891 + 1032 WP_006248514.1 tryptophan--tRNA ligase -
J450_RS05950 1227955..1228710 - 756 WP_006248513.1 M48 family metallopeptidase -
J450_RS05955 1228766..1229293 - 528 WP_006248512.1 inorganic diphosphatase -
J450_RS05970 1229772..1230809 + 1038 WP_041447997.1 endolytic transglycosylase MltG -
J450_RS05975 1230820..1231440 + 621 WP_006248508.1 dTMP kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-109)

Antitoxin

SpoVT-AbrB domain

(7-48)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12007.94 Da        Isoelectric Point: 6.4597

>T29687 WP_006248516.1 NC_021738:1226401-1226733 [Mannheimia haemolytica D171]
MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTV
DIAQRHGKFIEALPESLLDEIMARVSVIFE

Download         Length: 333 bp

>T29687 NC_021738:1226401-1226733 [Mannheimia haemolytica D171]
ATGAAACGTGGCGATATTTACTGGCTCGATCTTGATCCAACCTTAGGACACGAACAATCAGGCTTCCGCCCCGTTGTAAT
TGTTTCCGCAACTGCATTTAATCTAGCCAGTAAACTGCCTGTGGTTGTGCCGATTACTAACGGTGGAGCTTTTGCTGAAA
GACTGGGCTTTGCCGTTTCATTAAAAGATGCCGGCTTAAAAACAACAGGGATTATTCGATGCGATCAACCTCGCACAGTC
GATATTGCACAACGCCATGGAAAATTTATCGAAGCCTTACCCGAAAGCCTACTTGATGAAATTATGGCAAGGGTTTCAGT
GATTTTTGAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9418.88 Da        Isoelectric Point: 4.7228

>AT29687 WP_006248517.1 NC_021738:1226144-1226404 [Mannheimia haemolytica D171]
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL

Download         Length: 261 bp

>AT29687 NC_021738:1226144-1226404 [Mannheimia haemolytica D171]
ATGCCAACAATCGCATTACGTCAGGTCGGTGGCTCGGTTGCAATGGTGATTCCGCCTGCTTTTTTAGAGGCACTCAAGCT
CTCCGTCGGTAGCAAAGTGGATTTGTCTTTAAACGACAACCAACTCGTGATTAATCCTGCCCGCCCAAAAGTGAAATACA
GCTTAGAGGAATTATTAAGACAATCCGATTTTGAGGCAGCAAGGAATGAAGAAAGCCAATCGTGGTTATCCGAGCCGGCA
ATAGGAAAAGAAATTTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZY19

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZX26

References