Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 573431..574067 Replicon chromosome
Accession NZ_OX419570
Organism Bacillus subtilis isolate NRS6134

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag KJP59_RS03030 Protein ID WP_003156187.1
Coordinates 573717..574067 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag KJP59_RS03025 Protein ID WP_003225183.1
Coordinates 573431..573712 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJP59_RS03005 (569790) 569790..570389 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
KJP59_RS03010 (570484) 570484..570849 + 366 WP_003234281.1 holo-ACP synthase -
KJP59_RS03015 (571015) 571015..572031 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
KJP59_RS03020 (572146) 572146..573315 + 1170 WP_003234284.1 alanine racemase -
KJP59_RS03025 (573431) 573431..573712 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
KJP59_RS03030 (573717) 573717..574067 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KJP59_RS03035 (574182) 574182..575006 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
KJP59_RS03040 (575011) 575011..575376 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
KJP59_RS03045 (575380) 575380..575781 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
KJP59_RS03050 (575793) 575793..576800 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
KJP59_RS03055 (576862) 576862..577191 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
KJP59_RS03060 (577188) 577188..577670 + 483 WP_003234299.1 anti-sigma B factor RsbW -
KJP59_RS03065 (577636) 577636..578424 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
KJP59_RS03070 (578424) 578424..579023 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T296865 WP_003156187.1 NZ_OX419570:573717-574067 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT296865 WP_003225183.1 NZ_OX419570:573431-573712 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References