29685

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 892814..893454 Replicon chromosome
Accession NC_021738
Organism Mannheimia haemolytica D171

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag J450_RS04195 Protein ID WP_006250075.1
Coordinates 892814..892996 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NET2
Locus tag J450_RS04200 Protein ID WP_006250984.1
Coordinates 893035..893454 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J450_RS04160 888385..889431 - 1047 WP_020824125.1 conserved phage C-terminal domain-containing protein -
J450_RS04165 889428..890267 - 840 WP_006252350.1 Rha family transcriptional regulator -
J450_RS04170 890443..890703 - 261 WP_006252483.1 hypothetical protein -
J450_RS04175 890724..890924 - 201 WP_006248825.1 helix-turn-helix domain-containing protein -
J450_RS04180 891055..891738 + 684 WP_006252485.1 helix-turn-helix transcriptional regulator -
J450_RS04185 891813..892193 + 381 WP_006248823.1 hypothetical protein -
J450_RS04190 892198..892683 + 486 WP_006252741.1 hypothetical protein -
J450_RS04195 892814..892996 + 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
J450_RS04200 893035..893454 + 420 WP_006250984.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
J450_RS04205 893521..893826 - 306 WP_006250985.1 HigA family addiction module antidote protein -
J450_RS04210 893835..894110 - 276 WP_006250986.1 type II toxin-antitoxin system RelE/ParE family toxin -
J450_RS04215 894400..894630 + 231 WP_006250987.1 hypothetical protein -
J450_RS04220 895109..895432 - 324 WP_006250988.1 hypothetical protein -
J450_RS04225 895625..895810 + 186 WP_020824222.1 hypothetical protein -
J450_RS13090 895794..895958 + 165 WP_020824223.1 hypothetical protein -
J450_RS04230 895964..896422 + 459 WP_006253113.1 single-stranded DNA-binding protein -
J450_RS04235 896458..896817 + 360 WP_006250991.1 hypothetical protein -
J450_RS04240 896889..897692 + 804 WP_020824224.1 DUF2303 family protein -
J450_RS04245 897786..898220 + 435 WP_020824225.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 855722..903305 47583


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T29685 WP_006250075.1 NC_021738:892814-892996 [Mannheimia haemolytica D171]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T29685 NC_021738:892814-892996 [Mannheimia haemolytica D171]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15957.34 Da        Isoelectric Point: 4.6983

>AT29685 WP_006250984.1 NC_021738:893035-893454 [Mannheimia haemolytica D171]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQRFTILPH

Download         Length: 420 bp

>AT29685 NC_021738:893035-893454 [Mannheimia haemolytica D171]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATACCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGTAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACGCT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NET2

References