Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 518254..518890 Replicon chromosome
Accession NZ_OX419567
Organism Bacillus subtilis isolate NRS6107

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag KJP42_RS02640 Protein ID WP_003156187.1
Coordinates 518540..518890 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag KJP42_RS02635 Protein ID WP_003225183.1
Coordinates 518254..518535 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJP42_RS02615 (514613) 514613..515212 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
KJP42_RS02620 (515307) 515307..515672 + 366 WP_003234281.1 holo-ACP synthase -
KJP42_RS02625 (515838) 515838..516854 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
KJP42_RS02630 (516969) 516969..518138 + 1170 WP_003234284.1 alanine racemase -
KJP42_RS02635 (518254) 518254..518535 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
KJP42_RS02640 (518540) 518540..518890 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KJP42_RS02645 (519005) 519005..519829 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
KJP42_RS02650 (519834) 519834..520199 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
KJP42_RS02655 (520203) 520203..520604 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
KJP42_RS02660 (520616) 520616..521623 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
KJP42_RS02665 (521685) 521685..522014 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
KJP42_RS02670 (522011) 522011..522493 + 483 WP_003234299.1 anti-sigma B factor RsbW -
KJP42_RS02675 (522459) 522459..523247 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
KJP42_RS02680 (523247) 523247..523846 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T296836 WP_003156187.1 NZ_OX419567:518540-518890 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT296836 WP_003225183.1 NZ_OX419567:518254-518535 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References