Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 512225..512861 Replicon chromosome
Accession NZ_OX419565
Organism Bacillus subtilis isolate NRS6145

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag KJP64_RS02625 Protein ID WP_003156187.1
Coordinates 512511..512861 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag KJP64_RS02620 Protein ID WP_003225183.1
Coordinates 512225..512506 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJP64_RS02600 (NRS6145_02580) 508584..509183 - 600 WP_032722928.1 rhomboid family intramembrane serine protease -
KJP64_RS02605 (NRS6145_02585) 509278..509643 + 366 WP_015252768.1 holo-ACP synthase -
KJP64_RS02610 (NRS6145_02590) 509809..510825 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
KJP64_RS02615 (NRS6145_02595) 510940..512109 + 1170 WP_032722929.1 alanine racemase -
KJP64_RS02620 (NRS6145_02600) 512225..512506 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
KJP64_RS02625 (NRS6145_02605) 512511..512861 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KJP64_RS02630 (NRS6145_02610) 512977..513801 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
KJP64_RS02635 (NRS6145_02615) 513806..514171 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
KJP64_RS02640 (NRS6145_02620) 514175..514576 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
KJP64_RS02645 (NRS6145_02625) 514588..515595 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
KJP64_RS02650 (NRS6145_02630) 515657..515986 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
KJP64_RS02655 (NRS6145_02635) 515983..516465 + 483 WP_003234299.1 anti-sigma B factor RsbW -
KJP64_RS02660 (NRS6145_02640) 516431..517219 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
KJP64_RS02665 (NRS6145_02645) 517219..517818 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T296832 WP_003156187.1 NZ_OX419565:512511-512861 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT296832 WP_003225183.1 NZ_OX419565:512225-512506 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References