296808

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1330123..1331039 Replicon chromosome
Accession NZ_OX419560
Organism Bacillus subtilis isolate NRS6132

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag KJP75_RS06985 Protein ID WP_003244695.1
Coordinates 1330293..1331039 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag KJP75_RS06980 Protein ID WP_003232646.1
Coordinates 1330123..1330293 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJP75_RS06945 (NRS6132_06865) 1326989..1327315 + 327 WP_069703538.1 XkdW family protein -
KJP75_RS06950 (NRS6132_06870) 1327312..1327476 + 165 WP_014479563.1 XkdX family protein -
KJP75_RS06955 (NRS6132_06875) 1327520..1328359 + 840 WP_029727045.1 phage-like element PBSX protein XepA -
KJP75_RS06960 (NRS6132_06880) 1328412..1328681 + 270 WP_015252265.1 hemolysin XhlA family protein -
KJP75_RS06965 (NRS6132_06885) 1328694..1328957 + 264 WP_014479566.1 phage holin -
KJP75_RS06970 (NRS6132_06890) 1328970..1329863 + 894 WP_029727044.1 N-acetylmuramoyl-L-alanine amidase -
KJP75_RS06975 1329900..1330037 - 138 WP_119899277.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
KJP75_RS06980 (NRS6132_06895) 1330123..1330293 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
KJP75_RS06985 (NRS6132_06900) 1330293..1331039 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
KJP75_RS06990 (NRS6132_06905) 1331149..1332150 - 1002 WP_003232642.1 inorganic phosphate transporter -
KJP75_RS06995 (NRS6132_06910) 1332163..1332780 - 618 WP_003218470.1 DUF47 domain-containing protein -
KJP75_RS07000 (NRS6132_06915) 1333056..1334372 - 1317 WP_029317680.1 serine/threonine exchanger -
KJP75_RS07005 (NRS6132_06920) 1334761..1335711 + 951 WP_014476561.1 ring-cleaving dioxygenase -
KJP75_RS07010 1335820..1335915 + 96 Protein_1317 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T296808 WP_003244695.1 NZ_OX419560:c1331039-1330293 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT296808 WP_003232646.1 NZ_OX419560:c1330293-1330123 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References