296791

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1328432..1329348 Replicon chromosome
Accession NZ_OX419557
Organism Bacillus subtilis isolate NRS6186

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag KJP72_RS06980 Protein ID WP_003244695.1
Coordinates 1328602..1329348 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag KJP72_RS06975 Protein ID WP_003232646.1
Coordinates 1328432..1328602 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJP72_RS06940 (1325298) 1325298..1325624 + 327 WP_069703538.1 XkdW family protein -
KJP72_RS06945 (1325621) 1325621..1325785 + 165 WP_014479563.1 XkdX family protein -
KJP72_RS06950 (1325829) 1325829..1326668 + 840 WP_029727045.1 phage-like element PBSX protein XepA -
KJP72_RS06955 (1326721) 1326721..1326990 + 270 WP_015252265.1 hemolysin XhlA family protein -
KJP72_RS06960 (1327003) 1327003..1327266 + 264 WP_014479566.1 phage holin -
KJP72_RS06965 (1327279) 1327279..1328172 + 894 WP_029727044.1 N-acetylmuramoyl-L-alanine amidase -
KJP72_RS06970 (1328209) 1328209..1328346 - 138 WP_119899277.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
KJP72_RS06975 (1328432) 1328432..1328602 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
KJP72_RS06980 (1328602) 1328602..1329348 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
KJP72_RS06985 (1329458) 1329458..1330459 - 1002 WP_003232642.1 inorganic phosphate transporter -
KJP72_RS06990 (1330472) 1330472..1331089 - 618 WP_003218470.1 DUF47 domain-containing protein -
KJP72_RS06995 (1331365) 1331365..1332681 - 1317 WP_029317680.1 serine/threonine exchanger -
KJP72_RS07000 (1333070) 1333070..1334020 + 951 WP_014476561.1 ring-cleaving dioxygenase -
KJP72_RS07005 (1334129) 1334129..1334224 + 96 Protein_1316 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T296791 WP_003244695.1 NZ_OX419557:c1329348-1328602 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT296791 WP_003232646.1 NZ_OX419557:c1328602-1328432 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References