296789

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1361959..1362875 Replicon chromosome
Accession NZ_OX419555
Organism Bacillus subtilis isolate NRS6181

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag KJP65_RS07255 Protein ID WP_003244695.1
Coordinates 1362129..1362875 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag KJP65_RS07250 Protein ID WP_003232646.1
Coordinates 1361959..1362129 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJP65_RS07215 (NRS6181_07185) 1358825..1359151 + 327 WP_069703538.1 XkdW family protein -
KJP65_RS07220 (NRS6181_07190) 1359148..1359312 + 165 WP_014479563.1 XkdX family protein -
KJP65_RS07225 (NRS6181_07195) 1359356..1360195 + 840 WP_029727045.1 phage-like element PBSX protein XepA -
KJP65_RS07230 (NRS6181_07200) 1360248..1360517 + 270 WP_015252265.1 hemolysin XhlA family protein -
KJP65_RS07235 (NRS6181_07205) 1360530..1360793 + 264 WP_014479566.1 phage holin -
KJP65_RS07240 (NRS6181_07210) 1360806..1361699 + 894 WP_029727044.1 N-acetylmuramoyl-L-alanine amidase -
KJP65_RS07245 (NRS6181_07215) 1361736..1361873 - 138 WP_119899277.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
KJP65_RS07250 (NRS6181_07220) 1361959..1362129 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
KJP65_RS07255 (NRS6181_07225) 1362129..1362875 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
KJP65_RS07260 (NRS6181_07230) 1362985..1363986 - 1002 WP_003232642.1 inorganic phosphate transporter -
KJP65_RS07265 (NRS6181_07235) 1363999..1364616 - 618 WP_003218470.1 DUF47 domain-containing protein -
KJP65_RS07270 (NRS6181_07240) 1364892..1366208 - 1317 WP_029317680.1 serine/threonine exchanger -
KJP65_RS07275 (NRS6181_07245) 1366597..1367547 + 951 WP_014476561.1 ring-cleaving dioxygenase -
KJP65_RS07280 1367656..1367751 + 96 Protein_1371 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1326400..1371257 44857


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T296789 WP_003244695.1 NZ_OX419555:c1362875-1362129 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT296789 WP_003232646.1 NZ_OX419555:c1362129-1361959 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References