Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 517651..518287 Replicon chromosome
Accession NZ_OX419555
Organism Bacillus subtilis isolate NRS6181

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag KJP65_RS02640 Protein ID WP_003156187.1
Coordinates 517937..518287 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag KJP65_RS02635 Protein ID WP_003225183.1
Coordinates 517651..517932 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJP65_RS02615 (NRS6181_02600) 514010..514609 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
KJP65_RS02620 (NRS6181_02605) 514704..515069 + 366 WP_015252768.1 holo-ACP synthase -
KJP65_RS02625 (NRS6181_02610) 515235..516251 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
KJP65_RS02630 (NRS6181_02615) 516366..517535 + 1170 WP_003234284.1 alanine racemase -
KJP65_RS02635 (NRS6181_02620) 517651..517932 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
KJP65_RS02640 (NRS6181_02625) 517937..518287 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KJP65_RS02645 (NRS6181_02630) 518402..519226 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
KJP65_RS02650 (NRS6181_02635) 519231..519596 + 366 WP_015715277.1 RsbT antagonist protein RsbS -
KJP65_RS02655 (NRS6181_02640) 519600..520001 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
KJP65_RS02660 (NRS6181_02645) 520013..521020 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
KJP65_RS02665 (NRS6181_02650) 521082..521411 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
KJP65_RS02670 (NRS6181_02655) 521408..521890 + 483 WP_003234299.1 anti-sigma B factor RsbW -
KJP65_RS02675 (NRS6181_02660) 521856..522644 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
KJP65_RS02680 (NRS6181_02665) 522644..523243 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T296788 WP_003156187.1 NZ_OX419555:517937-518287 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT296788 WP_003225183.1 NZ_OX419555:517651-517932 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References