Detailed information of TA system TA029678

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 2599479..2600130
• Replicon: chromosome
• Accession: NC_021725
• Organism: Lactobacillus rhamnosus LOCK908

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: K8Q4Y0
• Locus tag: LOCK908_RS12030
• Protein ID: WP_005686631.1
• Coordinates: 2599479..2599862 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: K8QH19
• Locus tag: LOCK908_RS12035
• Protein ID: WP_005686632.1
• Coordinates: 2599882..2600130 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOCK908_RS12020	2598438..2598965	-	528	WP_005691221.1	QueT transporter family protein	-
LOCK908_RS12030	2599479..2599862	-	384	WP_005686631.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LOCK908_RS12035	2599882..2600130	-	249	WP_005686632.1	antitoxin	Antitoxin
LOCK908_RS12040	2600242..2601381	-	1140	WP_005691215.1	alanine racemase	-
LOCK908_RS12045	2601368..2601742	-	375	WP_005686634.1	holo-ACP synthase	-
LOCK908_RS12050	2601911..2603419	-	1509	WP_005686635.1	DEAD/DEAH box helicase	-
LOCK908_RS12055	2603693..2605081	-	1389	WP_014571607.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13757.03 Da
• Isoelectric Point: 10.9764

>T29678 WP_005686631.1 NC_021725:c2599862-2599479 [Lactobacillus rhamnosus LOCK908]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Nucleotide

• Length: 384 bp

>T29678 NC_021725:c2599862-2599479 [Lactobacillus rhamnosus LOCK908]
ATGGATGTAACAGTCAAACGCGGGGATGTATTTTTCGCCGATTTATCGCCGGTTGTCGGGTCGGAACAAGGCGGCAACCG
GCCCGTACTTATTATTCAAAACAATGTTGGTAACCATTATAGCCCCACTGTTATCGTGGCTGCCATTACCTCCAAAATCC
AAAAACCCAAAATGCCCACCCATGTCGGTTTGCGCGCCAAGCAAGACGGGGTTGAGCGTAATTCAGTCATTTTGCTTGAA
CAGATTCGTACAATTGATAAGCAACGGTTACAGGCACGCGTTACCGCTCTTTCAAGTGCCAAAATGGCAGCGGTGGATCG
GGCACTGGCGATATCGGTCGGACTCGTCAGCTTGCCTAAGCCGAAAACATATAATAAAAACTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8936.13 Da
• Isoelectric Point: 4.4778

>AT29678 WP_005686632.1 NC_021725:c2600130-2599882 [Lactobacillus rhamnosus LOCK908]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Nucleotide

• Length: 249 bp

>AT29678 NC_021725:c2600130-2599882 [Lactobacillus rhamnosus LOCK908]
ATGAAGAAGGAAACGTCAGTAACGGTCGCGCTTGATAGTGCTATCAAAGCGCGTTTGGATGCCTATTGCGAGTTGAATGA
TATGGGTGTTCAGGATGTCATTACTGAAGCAGTGGCGAAGTTTTTGCAGTCACAAGCACCTGAGATCAATCAATTGATTT
CTGGCTATGTGGCAATGGGACAAATCAATACGGAAATCAGCCATGCTTTCTCCGCCTGCGAAAGTGAGGCATACGCCCAT
ATTCGATAA

Structures

Toxin

• AlphaFold DB: K8Q4Y0

Antitoxin

• AlphaFold DB: K8QH19