296778

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1358351..1359267 Replicon chromosome
Accession NZ_OX419554
Organism Bacillus subtilis isolate NRS6160

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag KJP77_RS07225 Protein ID WP_003244695.1
Coordinates 1358521..1359267 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag KJP77_RS07220 Protein ID WP_003232646.1
Coordinates 1358351..1358521 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJP77_RS07185 (1355213) 1355213..1355542 + 330 WP_003232660.1 XkdW family protein -
KJP77_RS07190 (1355539) 1355539..1355703 + 165 WP_015715740.1 XkdX family protein -
KJP77_RS07195 (1355747) 1355747..1356586 + 840 WP_088467634.1 phage-like element PBSX protein XepA -
KJP77_RS07200 (1356639) 1356639..1356908 + 270 WP_015252265.1 hemolysin XhlA family protein -
KJP77_RS07205 (1356921) 1356921..1357184 + 264 WP_014479566.1 phage holin -
KJP77_RS07210 (1357197) 1357197..1358090 + 894 WP_195727878.1 N-acetylmuramoyl-L-alanine amidase -
KJP77_RS07215 (1358128) 1358128..1358265 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
KJP77_RS07220 (1358351) 1358351..1358521 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
KJP77_RS07225 (1358521) 1358521..1359267 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
KJP77_RS07230 (1359377) 1359377..1360378 - 1002 WP_014479569.1 inorganic phosphate transporter -
KJP77_RS07235 (1360391) 1360391..1361008 - 618 WP_003218470.1 DUF47 domain-containing protein -
KJP77_RS07240 (1361284) 1361284..1362600 - 1317 WP_015252262.1 serine/threonine exchanger -
KJP77_RS07245 (1362989) 1362989..1363939 + 951 WP_015715745.1 ring-cleaving dioxygenase -
KJP77_RS07250 (1364048) 1364048..1364143 + 96 Protein_1365 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T296778 WP_003244695.1 NZ_OX419554:c1359267-1358521 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT296778 WP_003232646.1 NZ_OX419554:c1358521-1358351 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References