Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 565930..566566 Replicon chromosome
Accession NZ_OX419554
Organism Bacillus subtilis isolate NRS6160

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag KJP77_RS02990 Protein ID WP_003156187.1
Coordinates 566216..566566 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag KJP77_RS02985 Protein ID WP_003225183.1
Coordinates 565930..566211 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJP77_RS02965 (562289) 562289..562888 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
KJP77_RS02970 (562983) 562983..563348 + 366 WP_003234281.1 holo-ACP synthase -
KJP77_RS02975 (563514) 563514..564530 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
KJP77_RS02980 (564645) 564645..565814 + 1170 WP_003234284.1 alanine racemase -
KJP77_RS02985 (565930) 565930..566211 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
KJP77_RS02990 (566216) 566216..566566 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KJP77_RS02995 (566681) 566681..567505 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
KJP77_RS03000 (567510) 567510..567875 + 366 WP_015715277.1 RsbT antagonist protein RsbS -
KJP77_RS03005 (567879) 567879..568280 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
KJP77_RS03010 (568292) 568292..569299 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
KJP77_RS03015 (569361) 569361..569690 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
KJP77_RS03020 (569687) 569687..570169 + 483 WP_003234299.1 anti-sigma B factor RsbW -
KJP77_RS03025 (570135) 570135..570923 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
KJP77_RS03030 (570923) 570923..571522 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T296777 WP_003156187.1 NZ_OX419554:566216-566566 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT296777 WP_003225183.1 NZ_OX419554:565930-566211 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References