Detailed information of TA system TA029677

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2502571..2503133
• Replicon: chromosome
• Accession: NC_021725
• Organism: Lactobacillus rhamnosus LOCK908

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: C2JYM4
• Locus tag: LOCK908_RS11560
• Protein ID: WP_005692155.1
• Coordinates: 2502571..2502918 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: LOCK908_RS11565
• Protein ID: WP_014571589.1
• Coordinates: 2502918..2503133 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOCK908_RS11540	2498066..2499307	-	1242	WP_005692160.1	acyltransferase	-
LOCK908_RS11545	2499304..2500005	-	702	WP_005692159.1	ABC transporter ATP-binding protein	-
LOCK908_RS11550	2499998..2500816	-	819	WP_014571588.1	ABC transporter permease subunit	-
LOCK908_RS11555	2501057..2501725	-	669	WP_005692157.1	tRNA ligase	-
LOCK908_RS14855	2501905..2502048	+	144	WP_020752296.1	hypothetical protein	-
LOCK908_RS11560	2502571..2502918	-	348	WP_005692155.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LOCK908_RS11565	2502918..2503133	-	216	WP_014571589.1	hypothetical protein	Antitoxin
LOCK908_RS11570	2503230..2505065	-	1836	WP_015764721.1	ABC transporter ATP-binding protein/permease	-
LOCK908_RS11575	2505052..2506842	-	1791	WP_015764722.1	ABC transporter ATP-binding protein/permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-90)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13150.01 Da
• Isoelectric Point: 7.9817

>T29677 WP_005692155.1 NC_021725:c2502918-2502571 [Lactobacillus rhamnosus LOCK908]
MTYKAKQGDVVWLDFDPSEGHEIRKRCPAVVLSSNAYNRATGFVIVAPITSTIRTLPGYVDIVSNKIRGQVVTSQVYSLD
VTEQGNRKIDFIERMNIEDFYTVAQFTKMNFDFKF

Nucleotide

• Length: 348 bp

>T29677 NC_021725:c2502918-2502571 [Lactobacillus rhamnosus LOCK908]
ATGACATATAAGGCTAAGCAAGGTGATGTGGTGTGGCTTGATTTTGATCCGTCCGAGGGTCATGAAATTAGAAAAAGATG
CCCAGCTGTTGTTTTAAGCAGTAACGCATACAATCGTGCAACCGGATTTGTGATTGTCGCACCAATTACCTCAACAATTC
GGACACTACCTGGTTATGTTGATATTGTATCCAATAAAATTCGTGGTCAGGTTGTCACTTCGCAGGTTTATTCTCTGGAT
GTCACTGAACAAGGCAACCGAAAAATCGATTTTATTGAAAGAATGAACATTGAAGATTTTTATACAGTCGCTCAATTCAC
CAAAATGAATTTTGATTTTAAATTTTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7986.13 Da
• Isoelectric Point: 5.7995

>AT29677 WP_014571589.1 NC_021725:c2503133-2502918 [Lactobacillus rhamnosus LOCK908]
MDVKVVKRGNSLALSLPSSAGFKRGEAFLLISDEKGGYLLIPKVKNPYTKAKPLSFHQEEAWPDFDYEDIE

Nucleotide

• Length: 216 bp

>AT29677 NC_021725:c2503133-2502918 [Lactobacillus rhamnosus LOCK908]
ATGGATGTGAAAGTTGTAAAACGTGGAAACTCGTTAGCTTTATCACTTCCGAGCAGTGCGGGTTTCAAACGAGGCGAGGC
GTTTTTGCTGATTTCAGATGAAAAAGGTGGTTATCTGTTGATCCCAAAGGTAAAAAATCCCTATACCAAAGCAAAACCAT
TATCATTCCATCAAGAAGAAGCTTGGCCGGATTTTGACTATGAGGATATTGAATAA

Structures

Toxin

• AlphaFold DB: A0A7Z9DS75