Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 519044..519680 Replicon chromosome
Accession NZ_OX419553
Organism Bacillus subtilis isolate NRS6183

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag KJP78_RS02645 Protein ID WP_003156187.1
Coordinates 519330..519680 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag KJP78_RS02640 Protein ID WP_003225183.1
Coordinates 519044..519325 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJP78_RS02620 (515404) 515404..516003 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
KJP78_RS02625 (516098) 516098..516463 + 366 WP_015252768.1 holo-ACP synthase -
KJP78_RS02630 (516629) 516629..517645 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
KJP78_RS02635 (517759) 517759..518928 + 1170 WP_029727188.1 alanine racemase -
KJP78_RS02640 (519044) 519044..519325 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
KJP78_RS02645 (519330) 519330..519680 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KJP78_RS02650 (519795) 519795..520619 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
KJP78_RS02655 (520624) 520624..520989 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
KJP78_RS02660 (520993) 520993..521394 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
KJP78_RS02665 (521406) 521406..522413 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
KJP78_RS02670 (522475) 522475..522804 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
KJP78_RS02675 (522801) 522801..523283 + 483 WP_003234299.1 anti-sigma B factor RsbW -
KJP78_RS02680 (523249) 523249..524037 + 789 WP_029727187.1 RNA polymerase sigma factor SigB -
KJP78_RS02685 (524037) 524037..524636 + 600 WP_003234303.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T296762 WP_003156187.1 NZ_OX419553:519330-519680 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT296762 WP_003225183.1 NZ_OX419553:519044-519325 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References