Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1330124..1331040 Replicon chromosome
Accession NZ_OX419550
Organism Bacillus subtilis isolate NRS6187

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag KJP69_RS06980 Protein ID WP_003244695.1
Coordinates 1330294..1331040 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag KJP69_RS06975 Protein ID WP_003232646.1
Coordinates 1330124..1330294 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJP69_RS06940 (NRS6187_06865) 1326990..1327316 + 327 WP_069703538.1 XkdW family protein -
KJP69_RS06945 (NRS6187_06870) 1327313..1327477 + 165 WP_014479563.1 XkdX family protein -
KJP69_RS06950 (NRS6187_06875) 1327521..1328360 + 840 WP_029727045.1 phage-like element PBSX protein XepA -
KJP69_RS06955 (NRS6187_06880) 1328413..1328682 + 270 WP_015252265.1 hemolysin XhlA family protein -
KJP69_RS06960 (NRS6187_06885) 1328695..1328958 + 264 WP_014479566.1 phage holin -
KJP69_RS06965 (NRS6187_06890) 1328971..1329864 + 894 WP_029727044.1 N-acetylmuramoyl-L-alanine amidase -
KJP69_RS06970 1329901..1330038 - 138 WP_119899277.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
KJP69_RS06975 (NRS6187_06895) 1330124..1330294 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
KJP69_RS06980 (NRS6187_06900) 1330294..1331040 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
KJP69_RS06985 (NRS6187_06905) 1331150..1332151 - 1002 WP_003232642.1 inorganic phosphate transporter -
KJP69_RS06990 (NRS6187_06910) 1332164..1332781 - 618 WP_003218470.1 DUF47 domain-containing protein -
KJP69_RS06995 (NRS6187_06915) 1333057..1334373 - 1317 WP_029317680.1 serine/threonine exchanger -
KJP69_RS07000 (NRS6187_06920) 1334762..1335712 + 951 WP_014476561.1 ring-cleaving dioxygenase -
KJP69_RS07005 1335821..1335916 + 96 Protein_1316 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T296753 WP_003244695.1 NZ_OX419550:c1331040-1330294 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT296753 WP_003232646.1 NZ_OX419550:c1330294-1330124 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References