Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 517830..518466 Replicon chromosome
Accession NZ_OX419550
Organism Bacillus subtilis isolate NRS6187

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag KJP69_RS02640 Protein ID WP_003156187.1
Coordinates 518116..518466 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag KJP69_RS02635 Protein ID WP_003225183.1
Coordinates 517830..518111 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJP69_RS02615 (NRS6187_02575) 514189..514788 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
KJP69_RS02620 (NRS6187_02580) 514883..515248 + 366 WP_015252768.1 holo-ACP synthase -
KJP69_RS02625 (NRS6187_02585) 515414..516430 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
KJP69_RS02630 (NRS6187_02590) 516545..517714 + 1170 WP_003234284.1 alanine racemase -
KJP69_RS02635 (NRS6187_02595) 517830..518111 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
KJP69_RS02640 (NRS6187_02600) 518116..518466 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KJP69_RS02645 (NRS6187_02605) 518581..519405 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
KJP69_RS02650 (NRS6187_02610) 519410..519775 + 366 WP_015715277.1 RsbT antagonist protein RsbS -
KJP69_RS02655 (NRS6187_02615) 519779..520180 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
KJP69_RS02660 (NRS6187_02620) 520192..521199 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
KJP69_RS02665 (NRS6187_02625) 521261..521590 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
KJP69_RS02670 (NRS6187_02630) 521587..522069 + 483 WP_003234299.1 anti-sigma B factor RsbW -
KJP69_RS02675 (NRS6187_02635) 522035..522823 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
KJP69_RS02680 (NRS6187_02640) 522823..523422 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T296752 WP_003156187.1 NZ_OX419550:518116-518466 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT296752 WP_003225183.1 NZ_OX419550:517830-518111 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References