Detailed information of TA system TA029675

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 2504655..2505306
• Replicon: chromosome
• Accession: NC_021723
• Organism: Lactobacillus rhamnosus LOCK900

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: K8Q4Y0
• Locus tag: LOCK900_RS11650
• Protein ID: WP_005686631.1
• Coordinates: 2504655..2505038 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: K8QH19
• Locus tag: LOCK900_RS11655
• Protein ID: WP_005686632.1
• Coordinates: 2505058..2505306 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOCK900_RS11645	2503716..2504243	-	528	WP_005711058.1	QueT transporter family protein	-
LOCK900_RS14420	2504281..2504478	-	198	WP_080565105.1	hypothetical protein	-
LOCK900_RS14425	2504445..2504658	+	214	Protein_2263	Malolactic regulator	-
LOCK900_RS11650	2504655..2505038	-	384	WP_005686631.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LOCK900_RS11655	2505058..2505306	-	249	WP_005686632.1	antitoxin	Antitoxin
LOCK900_RS11660	2505418..2506557	-	1140	WP_005710798.1	alanine racemase	-
LOCK900_RS11665	2506544..2506918	-	375	WP_005686634.1	holo-ACP synthase	-
LOCK900_RS11670	2507087..2508595	-	1509	WP_005686635.1	DEAD/DEAH box helicase	-
LOCK900_RS11675	2508869..2510257	-	1389	WP_005714110.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13757.03 Da
• Isoelectric Point: 10.9764

>T29675 WP_005686631.1 NC_021723:c2505038-2504655 [Lactobacillus rhamnosus LOCK900]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Nucleotide

• Length: 384 bp

>T29675 NC_021723:c2505038-2504655 [Lactobacillus rhamnosus LOCK900]
ATGGATGTAACAGTCAAACGCGGGGATGTATTTTTCGCCGATTTATCGCCGGTTGTCGGGTCGGAACAAGGCGGTAACCG
GCCCGTACTTATTATTCAAAACAATGTTGGTAACCATTATAGCCCCACTGTTATCGTGGCTGCCATTACCTCCAAAATCC
AAAAACCCAAAATGCCCACCCATGTCGGTTTGCGCGCCAAGCAAGACGGGGTTGAGCGTAATTCAGTCATTTTGCTTGAA
CAGATTCGTACAATTGATAAGCAACGGCTACAGGCACGCGTTACCGCTCTCTCAAGTGCCAAAATGGCAGCGGTGGATCG
GGCACTGGCGATATCGGTCGGACTCGTCAGCTTGCCTAAGCCGAAAACATATAATAAAAACTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8936.13 Da
• Isoelectric Point: 4.4778

>AT29675 WP_005686632.1 NC_021723:c2505306-2505058 [Lactobacillus rhamnosus LOCK900]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Nucleotide

• Length: 249 bp

>AT29675 NC_021723:c2505306-2505058 [Lactobacillus rhamnosus LOCK900]
ATGAAGAAGGAAACGTCAGTAACGGTCGCGCTTGATAGTGCTATCAAAGCGCGTTTGGATGCCTATTGCGAGTTGAATGA
TATGGGTGTTCAGGATGTCATTACTGAAGCAGTGGCGAAGTTTTTGCAGTCACAAGCACCTGAGATCAATCAATTGATTT
CTGGCTATGTGGCAATGGGACAAATCAATACGGAAATCAGCCATGCTTTCTCCGCCTGCGAAAGTGAGGCATACGCCCAT
ATTCGATAA

Structures

Toxin

• AlphaFold DB: K8Q4Y0

Antitoxin

• AlphaFold DB: K8QH19