Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2722191..2722842 Replicon chromosome
Accession NC_021721
Organism Lactobacillus casei LOCK919

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag LOCK919_RS13305 Protein ID WP_003567661.1
Coordinates 2722191..2722574 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag LOCK919_RS13310 Protein ID WP_003567665.1
Coordinates 2722594..2722842 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOCK919_RS13300 2721486..2722007 - 522 WP_003567657.1 QueT transporter family protein -
LOCK919_RS13305 2722191..2722574 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LOCK919_RS13310 2722594..2722842 - 249 WP_003567665.1 antitoxin Antitoxin
LOCK919_RS13315 2722910..2724046 - 1137 WP_003567667.1 alanine racemase -
LOCK919_RS13320 2724033..2724407 - 375 WP_003567669.1 holo-ACP synthase -
LOCK919_RS13325 2724576..2726084 - 1509 WP_013246091.1 DEAD/DEAH box helicase -
LOCK919_RS17065 2726210..2726353 - 144 WP_003567673.1 hypothetical protein -
LOCK919_RS13335 2726384..2727772 - 1389 WP_020751761.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T29673 WP_003567661.1 NC_021721:c2722574-2722191 [Lacticaseibacillus paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T29673 NC_021721:c2722574-2722191 [Lacticaseibacillus paracasei]
ATGGATGTGTCCGTAAAACGCGGCGATGTTTTTTTCGCCGATCTGTCTCCGGTTGTCGGCTCCGAACAAGGCGGCAACCG
GCCAGTACTTATTATTCAAAACAACGTTGGCAACCATTACAGTCCCACAGTGATTGTGGCAGCCATTACCTCCAAGATTC
AAAAACCCAAGATGCCGACGCATGTCGGGTTACGGGCCAAGCAAGATGGCGTTGAACGCAACTCGGTCATTTTACTTGAG
CAAATTCGCACGATTGACAAGCAACGGCTACAAGCCCGCGTGACAGCTCTGTCAAGCACCAAAATGGCAGCAGTCGACCG
CGCGTTGGCGATTTCAGTGGGCTTGGTCAGTTTGCCCAAGCCCAAAACATATAATAAGAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT29673 WP_003567665.1 NC_021721:c2722842-2722594 [Lacticaseibacillus paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT29673 NC_021721:c2722842-2722594 [Lacticaseibacillus paracasei]
ATGAAAAAGCAAACGTCAGTAGCGGTAACGTTTGATGATGCCATTAAGGCGCGGTTGGATGCCTATTGTGAATTGAATGA
CATTGACGCTCAGGCTGTGATTACGGAGGCAGTGGCGACATTTTTGAAGTCACAGGCTCCCGAAATCAACCAATTGATTT
CTGGCTATGTAGCAATGGGCCAGATCAATACAGAGATCAGTCATGCTTTCTCTGCCTGCGAGAGCGAGGCATACGCCCAT
ATTCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References