Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 3128236..3129046 Replicon chromosome
Accession NZ_OX359182
Organism Klebsiella pneumoniae strain KP34 isolate 2018-347802-001-01

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag OM989_RS15095 Protein ID WP_004178461.1
Coordinates 3128513..3129046 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag OM989_RS15090 Protein ID WP_002887278.1
Coordinates 3128236..3128502 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OM989_RS15070 (3124107) 3124107..3124451 - 345 WP_004894569.1 cation efflux system protein CusF -
OM989_RS15075 (3124469) 3124469..3125854 - 1386 WP_026005908.1 efflux transporter outer membrane subunit -
OM989_RS15080 (3126027) 3126027..3126710 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
OM989_RS15085 (3126700) 3126700..3128133 + 1434 WP_004186848.1 Cu(+)/Ag(+) sensor histidine kinase -
OM989_RS15090 (3128236) 3128236..3128502 + 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
OM989_RS15095 (3128513) 3128513..3129046 + 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
OM989_RS15100 (3129094) 3129094..3130215 - 1122 WP_004186850.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T296724 WP_004178461.1 NZ_OX359182:3128513-3129046 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT296724 WP_002887278.1 NZ_OX359182:3128236-3128502 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References