Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2432739..2433364 Replicon chromosome
Accession NZ_OX359182
Organism Klebsiella pneumoniae strain KP34 isolate 2018-347802-001-01

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag OM989_RS11805 Protein ID WP_002882817.1
Coordinates 2432981..2433364 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag OM989_RS11800 Protein ID WP_004150355.1
Coordinates 2432739..2432981 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OM989_RS11775 (2428730) 2428730..2429329 + 600 WP_004151865.1 glucose-1-phosphatase -
OM989_RS11780 (2429323) 2429323..2430183 + 861 WP_004146232.1 virulence factor BrkB family protein -
OM989_RS11785 (2430180) 2430180..2430617 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
OM989_RS11790 (2430662) 2430662..2431603 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
OM989_RS11795 (2431617) 2431617..2432534 - 918 WP_009484979.1 alpha/beta hydrolase -
OM989_RS11800 (2432739) 2432739..2432981 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
OM989_RS11805 (2432981) 2432981..2433364 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
OM989_RS11810 (2433538) 2433538..2434467 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
OM989_RS11815 (2434464) 2434464..2435099 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
OM989_RS11820 (2435096) 2435096..2435998 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T296719 WP_002882817.1 NZ_OX359182:2432981-2433364 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT296719 WP_004150355.1 NZ_OX359182:2432739-2432981 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References