Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5384383..5385008 Replicon chromosome
Accession NZ_OX359175
Organism Klebsiella pneumoniae strain KP47 isolate 2018-047100-007-01

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag ONI05_RS25975 Protein ID WP_002882817.1
Coordinates 5384383..5384766 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag ONI05_RS25980 Protein ID WP_004150355.1
Coordinates 5384766..5385008 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ONI05_RS25960 (5381749) 5381749..5382651 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
ONI05_RS25965 (5382648) 5382648..5383283 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
ONI05_RS25970 (5383280) 5383280..5384209 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
ONI05_RS25975 (5384383) 5384383..5384766 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
ONI05_RS25980 (5384766) 5384766..5385008 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
ONI05_RS25985 (5385213) 5385213..5386130 + 918 WP_009484979.1 alpha/beta hydrolase -
ONI05_RS25990 (5386144) 5386144..5387085 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
ONI05_RS25995 (5387130) 5387130..5387567 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
ONI05_RS26000 (5387564) 5387564..5388424 - 861 WP_004146232.1 virulence factor BrkB family protein -
ONI05_RS26005 (5388418) 5388418..5389017 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T296708 WP_002882817.1 NZ_OX359175:c5384766-5384383 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT296708 WP_004150355.1 NZ_OX359175:c5385008-5384766 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References