Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4726113..4726923 Replicon chromosome
Accession NZ_OX359175
Organism Klebsiella pneumoniae strain KP47 isolate 2018-047100-007-01

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag ONI05_RS22910 Protein ID WP_004178461.1
Coordinates 4726113..4726646 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag ONI05_RS22915 Protein ID WP_002887278.1
Coordinates 4726657..4726923 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ONI05_RS22905 (4724944) 4724944..4726065 + 1122 WP_004186850.1 cupin domain-containing protein -
ONI05_RS22910 (4726113) 4726113..4726646 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
ONI05_RS22915 (4726657) 4726657..4726923 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
ONI05_RS22920 (4727026) 4727026..4728459 - 1434 WP_004186848.1 Cu(+)/Ag(+) sensor histidine kinase -
ONI05_RS22925 (4728449) 4728449..4729132 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
ONI05_RS22930 (4729305) 4729305..4730690 + 1386 WP_026005908.1 efflux transporter outer membrane subunit -
ONI05_RS22935 (4730708) 4730708..4731052 + 345 WP_004894569.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T296703 WP_004178461.1 NZ_OX359175:c4726646-4726113 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT296703 WP_002887278.1 NZ_OX359175:c4726923-4726657 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References