Detailed information of TA system TA029661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1145571..1146133
• Replicon: chromosome
• Accession: NC_021715
• Organism: Mycobacterium yongonense 05-1390

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: S4Z4W9
• Locus tag: OEM_RS05660
• Protein ID: WP_020821856.1
• Coordinates: 1145765..1146133 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A081I0A4
• Locus tag: OEM_RS05655
• Protein ID: WP_036455532.1
• Coordinates: 1145571..1145768 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OEM_RS05620	1140677..1141822	-	1146	WP_201767262.1	hypothetical protein	-
OEM_RS05625	1141952..1143025	+	1074	WP_020821849.1	redox-regulated ATPase YchF	-
OEM_RS05630	1143042..1143782	+	741	WP_020821850.1	aminoglycoside 3'-phosphotransferase	-
OEM_RS05635	1143868..1144152	+	285	WP_020821851.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
OEM_RS05640	1144167..1144412	+	246	WP_041321307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
OEM_RS05645	1144448..1144999	+	552	WP_020821853.1	VOC family protein	-
OEM_RS05650	1145078..1145476	+	399	WP_020821854.1	VOC family protein	-
OEM_RS05655	1145571..1145768	+	198	WP_036455532.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
OEM_RS05660	1145765..1146133	+	369	WP_020821856.1	type II toxin-antitoxin system VapC family toxin	Toxin
OEM_RS05665	1146316..1146657	+	342	WP_020821857.1	hypothetical protein	-
OEM_RS27885	1146743..1147102	+	360	WP_201767263.1	hypothetical protein	-
OEM_RS05675	1147286..1147861	+	576	WP_075630262.1	C40 family peptidase	-
OEM_RS05680	1148224..1148547	+	324	WP_020821860.1	antibiotic biosynthesis monooxygenase	-
OEM_RS05685	1148587..1149420	-	834	WP_020821861.1	SAM-dependent methyltransferase	-
OEM_RS05690	1149455..1150138	-	684	WP_036429152.1	hypothetical protein	-
OEM_RS26265	1150285..1150494	+	210	WP_014381976.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13576.91 Da
• Isoelectric Point: 6.4873

>T29661 WP_020821856.1 NC_021715:1145765-1146133 [Mycobacterium intracellulare subsp. yongonense 05-1390]
MILVDSAVWIDHLHTAELRLVKLLEIDQVGCHALVIEELALGSILRREEVLDLLANLRQFPTVQHAEILHLTRQRKLYGR
GLSAVDVHLMAAVALVEGAQLWTRDKRLKAASVDAGIALFDA

Nucleotide

• Length: 369 bp

>T29661 NC_021715:1145765-1146133 [Mycobacterium intracellulare subsp. yongonense 05-1390]
ATGATCCTCGTCGACAGCGCGGTGTGGATCGATCATCTACACACCGCGGAATTGCGGCTAGTCAAGCTGCTGGAGATCGA
CCAAGTTGGCTGTCATGCGCTGGTCATCGAAGAGCTGGCGCTGGGGTCGATCTTGCGACGTGAGGAGGTGCTCGACCTCC
TCGCGAATCTTCGCCAGTTTCCGACCGTCCAGCACGCCGAGATCCTTCACCTCACCCGTCAACGCAAACTGTATGGACGG
GGTCTCAGTGCGGTCGACGTCCATCTCATGGCCGCCGTCGCCCTGGTTGAAGGCGCGCAGCTGTGGACGAGAGACAAGCG
GTTGAAGGCTGCGTCGGTCGATGCGGGCATCGCACTCTTCGATGCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6871.88 Da
• Isoelectric Point: 11.6072

>AT29661 WP_036455532.1 NC_021715:1145571-1145768 [Mycobacterium intracellulare subsp. yongonense 05-1390]
MRTTVTIDDDLLAKAAELTGVRENVALLREGLQTLIRVESARRLAALGGTDAGAVAAPRRRRGTG

Nucleotide

• Length: 198 bp

>AT29661 NC_021715:1145571-1145768 [Mycobacterium intracellulare subsp. yongonense 05-1390]
ATGCGGACCACAGTCACGATCGACGACGACCTGTTGGCCAAGGCTGCTGAGTTGACCGGCGTGCGCGAGAACGTCGCGTT
GCTCCGCGAGGGATTGCAGACGCTGATCAGGGTGGAAAGCGCTCGCCGACTTGCCGCGCTCGGCGGGACGGATGCCGGGG
CGGTGGCCGCTCCCCGGCGCCGCCGTGGGACTGGATGA

Structures

Toxin

• AlphaFold DB: S4Z4W9

Antitoxin

• AlphaFold DB: A0A081I0A4