296566

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 86259..86784 Replicon plasmid P1
Accession NZ_OX030729
Organism Escherichia coli isolate 68

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag LQ200_RS25800 Protein ID WP_001159868.1
Coordinates 86259..86564 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag LQ200_RS25805 Protein ID WP_000813634.1
Coordinates 86566..86784 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ200_RS25785 (82169) 82169..83335 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
LQ200_RS25790 (83923) 83923..84678 - 756 WP_000852146.1 replication initiation protein RepE -
LQ200_RS25795 (85452) 85452..86258 - 807 WP_000016982.1 site-specific integrase -
LQ200_RS25800 (86259) 86259..86564 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
LQ200_RS25805 (86566) 86566..86784 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LQ200_RS25810 (87418) 87418..87615 + 198 WP_000215657.1 hypothetical protein -
LQ200_RS25815 (87612) 87612..87896 - 285 WP_000642771.1 hypothetical protein -
LQ200_RS25820 (87916) 87916..89049 - 1134 WP_000545983.1 DUF3800 domain-containing protein -
LQ200_RS25825 (89312) 89312..90949 - 1638 WP_223201785.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(3)-IId / erm(B) / mph(A) / sul1 / qacE / aadA5 / aac(6')-Ib-cr / blaOXA-1 / catB3 / blaTEM-1B - 1..115409 115409


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T296566 WP_001159868.1 NZ_OX030729:c86564-86259 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT296566 WP_000813634.1 NZ_OX030729:c86784-86566 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References